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ASCP-MLT Questions and Answers

Question # 1

Yellow stopper tubes are used for all of the following except:

Question options:


human leukocyte antigen (HLA) typing


paternity testing


blood cultures


lead testing

Question # 2

Convert the following temperature from Celsius to Fahrenheit

-4 degrees C


14 degrees F


24.8 degrees F


27 degrees F


39.2 degrees F

Question # 3

Report the isolate as coagulase negative Staphylococcus is the correct answer because this is an isolate from a urine specimen with a coagulase negative Staphylococcus susceptible to novobiocin. Staphylococcus saprophyticus is resistant to novobiocin. Further testing is required to speciate coagulase negative Staphylococci but only if the specimen is from a sterile body site, not urine.

Gram positive cocci isolated from a catheterized urine culture on a 76-year-old male gave the following reactions:

Blood agar- creamy, white, opaque colonies

Catalase- positive

Slide coagulase- negative

Tube coagulase- negative

Novobiocin- susceptible

The next action the MLS should take is:


Report the isolate as coagulase negative staphylococcus


Report the isolate as Staphylococcus epidermidis


Report the isolate as Staphylococcus saprophyticus


Perform further testing to speciate the organism

Question # 4

Haemophilus influenzae is both X and V factor dependent for growth, and is the most common cause of obstructive laryngitis and epiglottitis. H. parainfluenzae is only V-factor dependent and causes only mild pharyngitis, rarely obstructive in nature. H. aphrophilus can be part of the normal upper respiratory flora in humans, does not cause pharyngitis, and is not X or V-factor dependent for growth. Although H. haemolyticus is both X and V-factor dependent, it is beta hemolytic on blood agar and is generally considered non pathogenic.

Acute obstructive epiglottitis, both in adults and children, is caused by the bacterial species shown in this split screen photograph. The colonies recovered on chocolate agar (upper frame) required both hematin and NAD, (as shown by colony growth only between the X and V strips in the lower frame). The most likely identification is:

ASCP-MLT question answer


Haemophilus parainfluenzae


Haemophilus influenzae


Haemophilus aphrophilus


Haemophilus haemolyticus

Question # 5

This is the lens power of the scanning objective lens.









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